Bhakti Pathak - ICMR-NIRRCH

Scientist F

Email: pathakb[at]nirrh[dot]res[dot]in
Phone: +(91-22) 2419-2015
Department: Cellular and Structural Biology

Research Interests

Cell biology
Cancer Biology
Protein-Protein interactions

Academic Background

M.Sc. University of Mumbai (Microbiology; 1996)
Ph.D. Cancer Research Institute, Tata Memorial Centre, Mumbai (Applied Biology; 2002)

Professional Experience

Sr No.	Institution/Place	Designation	From	To
1.	ICMR-NIRRCH	Scientist F	Sep 2020	Till date
2.	ICMR-NIRRH	Scientist E	Sep 2015	Aug 2020
3.	ICMR-NIRRH	Scientist D	Sept 2011	Aug 2015
4.	ICMR-NIRRH	Scientist C	Jan 2007	Aug 2011
5.	Wilson College, Mumbai	Lecturer, Microbiology and Biotechnology Dept	2005	2006
6.	Post-doctoral Fellow	Division of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, USA	April 2002	July 2005

Awards & Achievements

Publications

Projects

Research Staff

Publications

Mammalian cysteine-rich secretory proteins interact with plasma membrane Ca2+ exporter PMCA4b

2023

Miya V, Kumar C, Breed AA, Idicula-Thomas S, Pathak BR

Andrology, 2023 https://doi.org/10.1111/andr.13549

Mammalian cysteine-rich secretory proteins interact with plasma membrane Ca2+ exporter PMCA4b

2023

Miya V, Kumar C, Breed AA, Idicula-Thomas S, Pathak BR

Andrology, 2023, DOI: https://doi.org/10.1111/andr.13549

Comparison of Anti-Trop2 Extracellular Domain Antibodies Generated Against Peptide and Protein Immunogens for Targeting Trop2-Positive Tumour Cells.

2023

Kamble PR, Kulkarni B, Malaviya A, Bajaj M, Breed AA, Jagtap D, Mahale S, Pathak BR.

Appl Biochem Biotechnol. 2023 Sep 1.

Prognostic utility of the ovarian cancer secretome: a systematic investigation.

2022

Kamble PR, Breed AA, Pawar A, Kasle G, Pathak BR

Arch Gynecol Obstet 306(3): 639-662, 2022

Proteolytic cleavage of Trop2 at Arg87 is mediated by matriptase and regulated by Val194.

2020

Kamble PR, Rane S, Breed AA, Joseph S, Mahale SD, Pathak BR.

FEBS Lett 594(19): 3156-3169, 2020.

Androgen receptor mediated epigenetic regulation of CRISP3 promoter in prostate cancer cells.

2018

Pathak BR, Breed AA, Deshmukh P, Mahale SD.

J Steroid Biochem Mol Biol. 181: 20-27, 2018

Transcriptional regulation of follicle-stimulating hormone β-subunit in marmoset by an alternate distal promoter

2017

Kutteyil SS, Pathak BR, Mahale SD.

Gen Comp Endocrinol. 2017; S0016-6480(17)30009-6. doi: 10.1016/j.ygcen.2017.01.009

Functional characterization of two naturally occurring mutations V(221)G and T(449)N in the follicle stimulating hormone receptor.

2017

Banerjee AA, Achrekar SK, Joseph S, Pathak BR, Mahale SD.

Mol Cell Endocrinol. 2017 Jan 15;440:69-79. doi: 10.1016/j.mce.2016.11.020

Identification of CRISP2 from human sperm as PSP94 binding protein and generation of CRISP2 specific anti-peptide antibodies

2016

Anklesaria JH, Kulkarni BJ, Pathak BR, Mahale SD

J Pep Sci 22: 383-90, 2016

Cysteine-rich secretory protein 3 plays a role in prostate cancer cell invasion and affects expression of PSA and ANXA1

2016

Pathak BR, Breed AA, Apte S, Acharya K, Mahale SD

Mol Cell Biochem; 411:11-21, 2016

Comparison of marmoset and human FSH using synthetic peptides of the ?-subunit L2 loop region and anti-peptide antibodies

2016

Kutteyil SS, Kulkarni BJ, Mojidra R, Joseph S, Pathak B, Mahale SD

J Pept Sci 22: 397-405, 2016

Purification and characterization of CRISP-3 from human seminal plasma and its real-time binding kinetics with PSP94

2016

Anklesaria JH, Pandya RR1, Pathak BR, Mahale SD

J Chromatogr B Analyt Technol Biomed Life Sci, 2016

Cysteine-rich secretory protein 3 plays a role in prostate cancer cell invasion and affects expression of PSA and ANXA1

2015

Pathak BR, Breed AA, Apte S, Acharya K, Mahale SD

Mol Cell Biochem, 2015

Expression of Bioactive Callithrix jacchus Follicle-Stimulating Hormone in Pichiapastoris

2015

Kutteyil SS, Pathak BR, Dighe RR, Mahale SD

Appl Biochem Biotechnol 176:399-411, 2015

FSH receptor specific residues L501 and I505 in extracellular loop 2 are essential for its function

2015

Banerjee AA, Dupakuntla M, Pathak BR, Mahale SD

J Mol Endocrinol 54:193-204, 2015

Mapping of the binding sites involved in PSP94-CRISP-3 interaction by molecular dissection of the complex

2013

Breed AA, Gomes A, Roy BS, Mahale SD, Pathak BR

Biochim Biophys Acta Gen 1830: 3019-3029, 2013

Prostate secretory protein of 94 amino acids (PSP94) binds to prostatic acid phosphatase (PAP) in human seminal plasma

2013

Ankleseria JH, Jagtap DD, Pathak BR, Kadam KM, Joseph S, Mahale SD

PLoS One 8:e58631, 2013

Extracellular loop 2 in the FSH receptor is crucial for ligand mediated receptor activation

2012

Dupakuntla M, Pathak B, Roy BS, Mahale SD

Mol Cell Endocrinol 362:60-8, 2012

Is 37LRP cell surface receptor for PSP94?

2011

Pathak BR, Jagtap DD, Mahale SD

Asian J Androl 13:347, 2011

Follicle-Stimulating Hormone receptor polymorphism (G-29A) is associated with altered level of receptor expression in granulosa cells

2011

Desai SS, Achrekar SK, Pathak BR, Desai SK, Mangoli VS, Mangoli RV, Mahale SD

J Clin Endocrinol Metab 96, 2805-2812, 2

High affinity of seminal plasma PSP94 to human immunoglobulin is through the Fab domain

2010

Jagtap DD, Modi DN, Kumar M, Pathak BR, Mahale SD

Biochem Biophy Acta: Prot Proteom 1804: 1889-1894, 2010

Growth inhibition mediated by PSP94 or CRISP-3 is prostate cancer cell line specific

2010

Pathak BR, Breed AA, Nakhawa VH, Jagtap DD, Mahale SD

Asian J Androl 12: 677-689, 2010

Name	Designation
Ms. Pradnya Kamble	Ph.D. Student
Ms. Vaidehi Miya	PhD Student
Ms. Apoorva Pawar	PhD Student
Ms. Meghali Borkotoky	PhD Student
Ms. Jyoti Vishwakarma	PhD Student
Ms. Madhulika Bajaj	Project Associate

Name	Designation	From	To
Ms. Jagruti Gite	Trainee	Nov 2022	Feb 2023
Ms. Christine Fernandes	Trainee	Apr 2022	June 2022
Ms. Amish Malviya	Trainee	Dec 2021	May 2022
Ms. Shivali Patkar	JRF	2020	2021
Ms. Grishma Kasle	JRF	2019	2020
Ms. Sanjana Rane	JRF	2018	2020
Ms. Priya Mishra	JRF	2018	2019
Ms. Priti Kanade	JRF	2017	2017
Ms. Shreya Waghmode	JRF	2017	2017
Ms. Priyanka Deshmukh	JRF	2013	2014
Ms. Snehal Apte	JRF	2012	2013
Ms. Amanda Gomes	Project Assistant	2008	2011

Name of Award	Awarding agency	Year
International travel grant to attend 11th General Meeting of the International Proteolysis Society “Interfaces in Proteolysis””, Czech Republic	SERB	2019
Fast Track Grant for young Scientists	DST	2008
K S Krishnan Research Associateship (KSKRA)	BRNS DAE	2005 (Not availed)
International travel grant to attend 11th General Meeting of the International Proteolysis Society “Interfaces in Proteolysis””, Czech Republic	SERB	2019
“R H Jaju Award” for the Best Oral presentation	Indian Association for Cancer Research (IACR)	2014
Fast Track Grant for young Scientists	DST	2008
K S Krishnan Research Associateship (KSKRA)	BRNS DAE	2005 (Not availed)
Post-doctoral fellowship	Barshop Institute for Longevity and Aging Studies, UTHSCSA at San Antonio, USA	2002 – 2005
Junior and Senior Research fellowship from upon clearing the Joint UGC-CSIR National Eligibility Test (NET)	UGC	1997-2002
Cleared Maharashtra State Eligibility Test (MH-SET) for Assistant Professor (Microbiology)	Accredited by U.G.C.	1996

Project title	Funding agency	From	To
Evaluating the role of proteolytic processing of Trop1 and Trop2 in normal placentation and placental pathologies	DBT	2021	2024
Assessing Trop2 expression and its correlation with the anti-Trop2 immune status in ovarian cancer patients	Intramural (ICMR)	2016	2023
Analysis the molecular cargo of the extracellular vesicles secreted by ovarian cancer cells	Intramural (ICMR)	2021	2025

Project title	Funding agency	From	To
Deciphering the role of PSP94 and CRISP family proteins in ion-channel modulation	DST SERB	2018	2021
Evaluating the potential of Trop2 as immunotherapeutic target for ovarian cancer	Intramural (ICMR)	2015	2021
Developing an immunochromagraphy based strip test to analyze PLGF concentration in the urine for the risk of developing preeclampsia	DHR	2017	2019
Analyzing the effect and mechanism of CRISP-3 upregulation during prostate tumorigenesis	BRNS-DAE	2011	2014
Investigating the expression of Trop2 and its role in ovarian cancer stem cells (Mentor)	ICMR-PDF	2019	2020
Exploring the regulation and biological significance of alternative splicing of PSP94	DST	2008	2011

Bhakti Pathak - ICMR-NIRRCH

Scientist F

Research Interests

Academic Background

Professional Experience

Present

Past

Awards & Achievements

Publications

Mammalian cysteine-rich secretory proteins interact with plasma membrane Ca2+ exporter PMCA4b

Mammalian cysteine-rich secretory proteins interact with plasma membrane Ca2+ exporter PMCA4b

Comparison of Anti-Trop2 Extracellular Domain Antibodies Generated Against Peptide and Protein Immunogens for Targeting Trop2-Positive Tumour Cells.

Prognostic utility of the ovarian cancer secretome: a systematic investigation.

Proteolytic cleavage of Trop2 at Arg87 is mediated by matriptase and regulated by Val194.

Androgen receptor mediated epigenetic regulation of CRISP3 promoter in prostate cancer cells.

Transcriptional regulation of follicle-stimulating hormone β-subunit in marmoset by an alternate distal promoter

Functional characterization of two naturally occurring mutations V(221)G and T(449)N in the follicle stimulating hormone receptor.

Identification of CRISP2 from human sperm as PSP94 binding protein and generation of CRISP2 specific anti-peptide antibodies

Cysteine-rich secretory protein 3 plays a role in prostate cancer cell invasion and affects expression of PSA and ANXA1

Comparison of marmoset and human FSH using synthetic peptides of the ?-subunit L2 loop region and anti-peptide antibodies

Purification and characterization of CRISP-3 from human seminal plasma and its real-time binding kinetics with PSP94

Cysteine-rich secretory protein 3 plays a role in prostate cancer cell invasion and affects expression of PSA and ANXA1

Expression of Bioactive Callithrix jacchus Follicle-Stimulating Hormone in Pichiapastoris

FSH receptor specific residues L501 and I505 in extracellular loop 2 are essential for its function

Mapping of the binding sites involved in PSP94-CRISP-3 interaction by molecular dissection of the complex

Prostate secretory protein of 94 amino acids (PSP94) binds to prostatic acid phosphatase (PAP) in human seminal plasma

Extracellular loop 2 in the FSH receptor is crucial for ligand mediated receptor activation

Is 37LRP cell surface receptor for PSP94?

Follicle-Stimulating Hormone receptor polymorphism (G-29A) is associated with altered level of receptor expression in granulosa cells

High affinity of seminal plasma PSP94 to human immunoglobulin is through the Fab domain

Growth inhibition mediated by PSP94 or CRISP-3 is prostate cancer cell line specific

Projects Ongoing

Projects Completed